Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid

Quercetin-3-glucuronide (Q3GA), the main phase II metabolite of quercetin (Q) in human plasma, is considered to be a more stable form Q for transport with bloodstream tissues, where it can potentially deconjugated by β-glucuronidase (β-Gluc) aglycone, which easily enters brain. This study evaluates effect lipopolysaccharide (LPS)-induced acute inflammation on β-Gluc gene expression choroid plexus (ChP) and its activity blood ChP cerebrospinal fluid (CSF), concentration metabolites plasma CSF. Studies were performed saline- LPS-treated adult ewes (n = 40) receiving Q3GA intravenously 16) primary rat epithelial cells papilloma cells. We observed that stimulated but not CSF, did affect concentrations except Q3GA, was higher 30 min after administration (p < 0.05) LPS- compared saline-treated ewes. The lack deconjugation under physiological inflammatory conditions, however, does exclude possible role course neurodegenerative diseases.